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KMID : 0379119970250020143
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Korean Journal of Mycology
1997 Volume.25 No. 2 p.143 ~ p.151
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Development of Lipase Hyper - producing Strain from Hybrids between Aspergillus niger and Penicillium notatum by Nuclear Transfer
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Abstract
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Interspecific hybrids between Aspergillns niger and Penicillium notatum (Tyr-), hyperlipolytic enzyne-producing fungi, were obtained by nuclear transfer technique. Optimal conditions for formation of intergeneric hybrids were investigated. Maximum production of protoplasts was obtained by 1 % Novozyme 234 at 30℃ for 3 hrs and the most effective osmotic stabilizers for the isolation of protoplasts were 0.6 M KCl. Frequencies of hybrid formation by nuclear transfer were 3.8×10^(-3)∼1.3×10^(-3). From the observation of genetic stability, conidial size, DNA content, and nuclear stain, it was suggested that their karyotypes are aneuploid. 1fie hybrids showed 1.2∼1.7 fold higher lipase activities than parental strains. It was strongly supported by results of this study that nuclear transfer technique is much more efficient in the formation of intergeneric hybrids than protoplast fusion and is very useful for the improvement of strains.
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KEYWORD
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